Investigation of Various Aminoglycoside Resistant Genes in Providencia Isolates

Ebru ATAYETER; Mahmut BAYKAN

doi:10.5281/zenodo.7361124

Authors

Ebru ATAYETER Medical Microbiology, Necmettin Erbakan University, Turkey https://orcid.org/0000-0003-4993-6785
Mahmut BAYKAN Bilecik Şeyh Edebali University, Turkey https://orcid.org/0000-0002-9954-6040

DOI:

https://doi.org/10.5281/zenodo.7361124

Keywords:

Providencia stuartii, multiple drug resistance, 16S rRNA methyltransferase, aminoglycoside resistance

Abstract

Abstract

Providencia species cause severe nosocomial infections with multi-drug resistance, especially in people who are hospitalized for a long time. This study aimed to determine the presence of aminoglycoside drug resistance genes that are part of multidrug resistance in Providencia species.

The strains that reproduced Providencia spp from the biological material sent to Necmettin Erbakan University Meram Medical Faculty Medical Microbiology Laboratory and were resistant to aminoglycoside drugs were included in our study. PCR method was used to determine the genes causing drug resistance at the molecular level and the presence of 33 genes was investigated.

As a result of our research, seven aminoglycoside modifying enzyme genes (aac (2 ") - Ia, aac (3) -IIa, aac (3) -IIc, aac (6 ') - Ib, aac (6') - Ib-cr, ant (2 ') - Ia, ant (3 ") - Ia) and one 16S rRNA methylation gene (armA) a total of eight genes were found to be positive in combination. In addition, ESBL positivity was detected in our isolates.
To the best of our knowledge, our study is the first study in Turkey in which the aminoglycoside resistance gene as well as the armA and aac (6') - Ib-cr gene were positively detected in Providencia species.

References

&Gazi, H. Ermertcan Ş. Hoşgör-Limoncu M. Taşlı H. Eraç B. 2008. “Gram-Negatif Bakterilerde Plazmit Aracılı Y{ü}ksek D{ü}zey Aminoglikozit Direncinin Araştırılması.” İnfeksiyon Dergisi (Turkish Journal of Infection). 2008. https://avesis.ege.edu.tr/yayin/2cbf7b83-3d8b-4ee2-a4b4-560f64f5d36b/gram-negatif-bakterilerde-plazmit-aracili-yuksek-duzey-aminoglikozit-direncinin-arastirilmasi.

Akalin, H. Erdal, Mustafa Torun, and Ruhi Alacam. 1988. “Aminoglycoside Resistance Patterns in Turkey.” Scandinavian Journal of Infectious Diseases 20 (2): 199–203. https://doi.org/10.3109/00365548809032438.

Arpin, C., L. Thabet, H. Yassine, A. A. Messadi, J. Boukadida, V. Dubois, L. Coulange-Mayonnove, C. Andre, and C. Quentina. 2012.

“Evolution of an Incompatibility Group IncA/C Plasmid Harboring Bla CMY-16 and QnrA6 Genes and Its Transfer through Three Clones of Providencia Stuartii during a Two-Year Outbreak in a Tunisian Burn Unit.” Antimicrobial Agents and Chemotherapy 56 (3): 1342–49. https://doi.org/10.1128/AAC.05267-11.

Aubert, Daniel, Thierry Naas, Marie Frédérique Lartigue, and Patrice Nordmann. 2005. “Novel Genetic Structure Associated with an Extended-Spectrum β-Lactamase BlaVEB Gene in a Providencia Stuartii Clinical Isolate from Algeria.” Antimicrobial Agents and Chemotherapy 49 (8): 3590–92. https://doi.org/10.1128/AAC.49.8.3590-3592.2005.

Bercot, Béatrice, Laurent Poirel, and Patrice Nordmann. 2011. “Updated Multiplex Polymerase Chain Reaction for Detection of 16S RRNA Methylases: High Prevalence among NDM-1 Producers.” Diagnostic Microbiology and Infectious Disease 71 (4): 442–45. https://doi.org/10.1016/j.diagmicrobio.2011.08.016.

Cirit, Osman Sezer, Marta Fernández-Martínez, Buket Yayla, and Luis Martínez-Martínez. 2019. “Aminoglycoside Resistance Determinants in Multiresistant Escherichia Coli and Klebsiella Pneumoniae Clinical Isolates from Turkish and Syrian Patients.” Acta Microbiologica et Immunologica Hungarica 66 (3): 327–35. https://doi.org/10.1556/030.66.2019.005.

Costello, Sarah E., Lalitagauri M. Deshpande, Andrew P. Davis, Rodrigo E. Mendes, and Mariana Castanheira. 2019. “Aminoglycoside-Modifying Enzyme and 16S Ribosomal RNA Methyltransferase Genes among a Global Collection of Gram-Negative Isolates.” Journal of Global Antimicrobial Resistance 16 (March): 278–85. https://doi.org/10.1016/j.jgar.2018.10.020.

Doi, Yohei, and Yoshichika Arakawa. 2007. “16S Ribosomal RNA Methylation: Emerging Resistance Mechanism against Aminoglycosides.” Clinical Infectious Diseases. Clin Infect Dis. https://doi.org/10.1086/518605.

Ermertcan, Şafak, Fethiye Ferda Yılmaz, Hüseyin Taşlı, and Ayşe Nur Yurtma. 2013. “Aminoglikozit Dirençli Gram Negatif Bakterilerde Plazmid Aracılı Metilaz Genlerinin Araştırılması.” Türk Mikrobiyol Cem Derg 43 (1): 12–16.

EUCAST. n.d. “Natural Resistance and Unexpected Phenotypes Expert Rules Release.” EUCAST. Accessed May 23, 2021.

https://www.tmc-online.org/userfiles/file/EUCAST_Uzman_Kurallari_Surum_3_1.pdf.

Galimand, Marc, Patrice Courvalin, and Thierry Lambert. 2003. “Plasmid-Mediated High-Level Resistance to Aminoglycosides in Enterobacteriaceae Due to 16S RRNA Methylation.” Antımıcrobıal Agents And Chemotherapy 47 (8): 2565–71. https://doi.org/10.1128/AAC.47.8.2565-2571.2003.

Galimand. 2012. “RmtF, a New Member of the Aminoglycoside Resistance 16S RRNA N7 G1405 Methyltransferase Family.” https://doi.org/10.1128/AAC.00660-12.

Gokmen, Tulin Güven, Togrul Nagiyev, Melda Meral, Cansu Onlen, Farzad Heydari, and Fatih Koksal. 2016. “NDM-1 and Rmtc-Producing Klebsiella Pneumoniae Isolates in Turkey.” Jundishapur Journal of Microbiology 9 (10). https://doi.org/10.5812/jjm.33990.

Gür, Deniz, G. Hascelik, N. Aydin, M. Telli, M. Gültekin, D. Ögünç, O. A. Arikan, et al. 2009. “Antimicrobial Resistance in Gram-Negative Hospital Isolates: Results of the Turkish HITIT-2 Surveillance Study of 2007.” Journal of Chemotherapy 21 (4): 383–89. https://doi.org/10.1179/joc.2009.21.4.383.

Gür, Deniz, Ufuk Hasdemir, Aslı Çakar, İffet Çavuşoğlu, Tuğçe Çelik, and Burak Aksu. 2020. “Comparative in Vitro Activity of Plazomicin and Older Aminoglyosides against Enterobacterales Isolates; Prevalence of Aminoglycoside Modifying Enzymes and 16S RRNA Methyltransferases.” Diagnostic Microbiology and Infectious Disease 97 (4). https://doi.org/10.1016/j.diagmicrobio.2020.115092.

Hayakawa, Kayoko, Dror Marchaim, George W. Divine, Jason M. Pogue, Sarwan Kumar, Paul Lephart, Ken Risko, Jack D. Sobel, and Keith S. Kaye. 2012. “Growing Prevalence of Providencia Stuartii Associated with the Increased Usage of Colistin at a Tertiary Health Care Center.” International Journal of Infectious Diseases. Int J Infect Dis. https://doi.org/10.1016/j.ijid.2012.05.1029.

Jacoby, George A. 2009. “AmpC Β-Lactamases.” Clinical Microbiology Reviews. Clin Microbiol Rev. https://doi.org/10.1128/CMR.00036-08.

Jarlier, Vincent, Marie Helene Nicolas, Genevieve Fournier, and Alain Philippon. 1988. “Extended Broad-Spectrum β-Lactamases Conferring Transferable Resistance to Newer β-Lactam Agents in Enterobacteriaceae: Hospital Prevalence and Susceptibility Patterns.” Clinical Infectious Diseases 10 (4): 867–78. https://doi.org/10.1093/clinids/10.4.867.

Kim, Yun Tae, Ji Hyun Jang, Hyun Chul Kim, Hyogyeong Kim, Kyoung Ryul Lee, Kyung Sun Park, Hee Joo Lee, and Young Jin Kim. 2011. “Identification of Strain Harboring Both Aac(6’)-Ib and Aac(6’)-Ib-Cr Variant Simultaneously in Escherichia Coli and Klebsiella Pneumoniae.” BMB Reports 44 (4): 262–66. https://doi.org/10.5483/BMBRep.2011.44.4.262.

Kraker, M. E.A. de, V. Jarlier, J. C.M. Monen, O. E. Heuer, N. van de Sande, and H. Grundmann. 2013. “The Changing Epidemiology of Bacteraemias in Europe: Trends from the European Antimicrobial Resistance Surveillance System.” Clinical Microbiology and Infection 19 (9): 860–68. https://doi.org/10.1111/1469-0691.12028.

Krause, Kevin M, Alisa W Serio, Timothy R Kane, and Lynn E Connolly. n.d. “Aminoglycosides: An Overview.” https://doi.org/10.1101/cshperspect.a027029.

Macinga, D. R., and P. N. Rather. 1999. “The Chromosomal 2’-N-Acetyltransferase of Providencia Stuartii: Physiological Functions and Genetic Regulation.” Frontiers in Bioscience : A Journal and Virtual Library. Front Biosci. https://doi.org/10.2741/macinga.

Mingeot-Leclercq, Marie-Paule, Youri Glupczynski, and Paul M Tulkens. 1999. “Aminoglycosides: Activity and Resistance.” Vol. 43. http://aac.asm.org/.

Nazic, Hasan, Laurent Poirel, and Patrice Nordmann. 2005. “Further Identification of Plasmid-Mediated Quinolone Resistance Determinant in Enterobacteriaceae in Turkey [4].” Antimicrobial Agents and Chemotherapy. American Society for Microbiology (ASM). https://doi.org/10.1128/AAC.49.5.2146-2147.2005.

Över, Ufuk, Deniz Gür, Serhat ÜnaL, and George H. Mıller. 2000. “Gram-Negatif Bakterilerde Aminoglikozid Antibiyotiklere Karşı Direnç Mekanizmaları: Son Gelişmeler ve Türkiye Sonuçları.” Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi 5 (3): 168–78. https://app.trdizin.gov.tr/makale/TVRBMk1URXg/gram-negatif-bakterilerde-aminoglikozid-antibiyotiklere-karsi-direnc-mekanizmalari-son-gelismeler-ve-turkiye-sonuclari.

Park, Yeon-Joon. 2009. “Aminoglycoside Resistance in Gram-Negative Bacilli.” Korean Journal of Clinical Microbiology 12 (2): 57. https://doi.org/10.5145/kjcm.2009.12.2.57.

Robicsek, A., Strahilevitz, J., Jacob, G. A., Macielag, M., Abbanat, D., Park, C. H. et. al. 2006. “Fluoroquinolone-Modifying Enzyme: A New Adaptation of a Common Aminoglycoside Acetyltransferase.” Nat Med 12 (1): 83–88.

Strahilevitz, Jacob, George A. Jacoby, David C. Hooper, and Ari Robicsek. 2009. “Plasmid-Mediated Quinolone Resistance: A Multifaceted Threat.” Clinical Microbiology Reviews. Clin Microbiol Rev. https://doi.org/10.1128/CMR.00016-09.

Vanhoof, R., Hannecart-Pokorni, E., & Content, J. 1998. “Nomenclature of Genes Encoding Aminoglycoside-Modifying Enzymes.” Antimicrob Agents Chemother 42 (2): 483.

Wachino, Jun Ichi, and Yoshichika Arakawa. 2012. “Exogenously Acquired 16S RRNA Methyltransferases Found in Aminoglycoside-Resistant Pathogenic Gram-Negative Bacteria: An Update.” Drug Resistance Updates 15 (3): 133–48.

https://doi.org/10.1016/j.drup.2012.05.001.

Wie, Seong-Heon. 2015. “Clinical Significance of Providencia Bacteremia or Bacteriuria.” Korean J Intern Med 30: 167–69. https://doi.org/10.3904/kjim.2015.30.2.167.

Investigation of Various Aminoglycoside Resistant Genes in Providencia Isolates

Authors

DOI:

Keywords:

Abstract

References

Downloads

Published

How to Cite

Issue

Section

License

Make a Submission

Language

Information

Current Issue