Investigation of Various Aminoglycoside Resistant Genes in Providencia Isolates
Özet Görüntüleme: 158 / PDF İndirme: 106
DOI:
https://doi.org/10.5281/zenodo.7361124Anahtar Kelimeler:
Providencia stuartii- çoklu ilaç direnci- 116S rRNA metiltransferaz- aminoglikozid direnciÖzet
Özet
Providencia türleri, özellikle uzun süre hastanede yatan kişilerde çoklu ilaç direnci ile ciddi nozokomiyal enfeksiyonlara neden olmaktadır. Bu çalışma, Providencia türlerinde çoklu ilaç direncinin bir parçası olan aminoglikozid ilaç direnç genlerinin varlığını belirlemeyi amaçlamıştır .
Çalışmamıza Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi Tıbbi Mikrobiyoloji Laboratuvarı'na gönderilen biyolojik materyalden Providencia spp üreyen ve aminoglikozit ilaçlara dirençli suşlar dahil edildi. İlaç direncine neden olan genlerin moleküler düzeyde belirlenmesi için PCR yöntemi kullanılmış ve 33 genin varlığı araştırılmıştır.
Araştırmamız sonucunda yedi aminoglikozit modifiye edici enzim geni ( aac (2") - Ia, aac (3) -IIa, aac (3) -IIc, aac (6') - Ib, aac (6') - Ib -cr, ant (2') - Ia, ant (3") - Ia ) ve bir adet 16S rRNA metilasyon geni ( kol A) kombinasyonunda toplam sekiz genin pozitif olduğu bulundu. Ayrıca izolatlarımızda GSBL pozitifliği saptanmıştır. Bildiğimiz kadarıyla çalışmamız, Providencia türlerinde aminoglikozid direnç geni ile armA ve aac (6') - Ib-cr geninin pozitif olarak saptandığı
Türkiye'deki ilk çalışmadır .
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